Cover your freeze dryer trays with parchment paper to prevent sticking, or use the silicone cube molds sold by Harvest Right.
Place your trays on a flat surface, and fill trays ¾ high with your starter culture. (If using silicone molds, fill them to the top.)
Pre-freeze the trays of starter culture in a freezer overnight for optimal energy efficiency and to save wear and tear on your freeze drying unit.
Pre-chill your freeze drier for 20 minutes before adding frozen trays of starter culture.
Slide each tray back into the freeze dryer, put the insulating cover in place, close the door, lock down the pump and press start. (This step takes about 24 hours)
After the freeze drying process is complete, remove the trays and package the finished product for long-term storage.